Reagents required but not supplied – Bio-Rad Bio-Plex Pro™ TGF-β Assays User Manual
Page 7

5
Reagents Required but Not Supplied
TGF-b is secreted as part of a complex that causes it to be inactive.
It is necessary to expose samples to acidic conditions in order to activate
TGF-b. The following reagents are required:
n
1 N hydrochloric acid: To make 100 ml of 1 N HCl, add 8.33 ml of
12 N HCl slowly to 91.67 ml of deionized water, and mix well
n
1.2 N sodium hydroxide/0.5 M HEPES: To make 100 ml of 1.2
NaOH/0.5 M HEPES, add 12 ml of 10 N NaOH to 75 ml of deionized
water, and mix well. Add 11.9 g of HEPES (free acid, MW 238.3), mix
well, and bring the final volume to 100 ml with deionized water
See also other documents in the category Bio-Rad Accessories for water:
- TransFectin™ Lipid Reagent (2 pages)
- Gene Pulser MXcell™ Electroporation System (66 pages)
- Gene Pulser MXcell™ Electroporation System (19 pages)
- Gene Pulser Xcell™ Electroporation Systems (83 pages)
- Gene Pulser® Electroporation Buffer (2 pages)
- MicroPulser™ Electroporator (31 pages)
- Helios® Gene Gun System (52 pages)
- PDS-1000 / He™ and Hepta™ Systems (51 pages)
- TGX™ FastCast™ Acrylamide Solutions (2 pages)
- Criterion™ TGX Stain-Free™ Precast Gels (16 pages)
- Gel Doc™ EZ System (22 pages)
- Criterion Stain Free™ Tris-HCl Gels (96 pages)
- Image Lab™ Software (212 pages)
- Mini-PROTEAN® TGX™ Precast Gels (52 pages)
- ChemiDoc™ XRS+ System (4 pages)
- ChemiDoc™ XRS+ System (50 pages)
- GS-800™ Calibrated Densitometer (444 pages)
- ChemiDoc™ MP System (8 pages)
- Image Lab™ Software (236 pages)
- ChemiDoc™ XRS+ System (42 pages)
- Criterion™ TGX™ Precast Gels (60 pages)
- Criterion™ Cell (13 pages)
- Image Lab™ Software (260 pages)
- Criterion™ XT Bis-Tris Precast Gels (26 pages)
- 2-D Electrophoresis Workflow (14 pages)
- 2-D Electrophoresis Workflow (22 pages)
- 2-D Electrophoresis Workflow (20 pages)
- PROTEAN® i12™ IEF System (4 pages)
- ReadyPrep™ 2-D Starter Kit (28 pages)
- EXQuest Spot Cutter (81 pages)
- Fluorescent Protein Stains (27 pages)
- Ready Gel® Zymogram Precast Gels (46 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (44 pages)
- Precision Plus Protein™ Prestained Standards (3 pages)
- Precision Plus Protein™ Unstained Standards (16 pages)
- Prestained SDS-PAGE Standards (3 pages)
- Silver Stains (20 pages)
- Unstained SDS-PAGE Standards (3 pages)
- Biotinylated Standards (3 pages)
- Biotinylated Standards (11 pages)
- IEF and 2-D Standards (3 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (20 pages)
- PROTEAN® Plus Hinged Spacer Plates and Combs (21 pages)
- Mini-PROTEAN® Tetra Handcast Systems (10 pages)
- Mini-PROTEAN 3 Multi-Casting Chamber (10 pages)