beautypg.com

Ez load precision molecular mass standard – Bio-Rad Molecular Mass Rulers User Manual

Page 8

background image

Fig. 6. 5 µl of the EZ Load Precision Molecular Mass Standard was
loaded onto a 1.8% Molecular Biology Certified agarose gel.
The gel was
run at 70 V for 75 minutes in 1x TBE buffer. The gel was stained in EtBr for
15 minutes and destained in dH

2

O for 30 minutes.

1 3

EZ Load Precision Molecular Mass
Standard

Catalog Number 170-8356

Use

Load 5 µl per lane. Adjustments may be made to
the loading volume for different well sizes and
desired band intensity. The EZ Load Precision
Molecular Mass Standard can be resolved in
agarose gels of up to 2% and polyacrylamide
gels up to 8%.

When used at 5 µl per lane:
1,000 bp

100 ng

700 bp

70 ng

500 bp

50 ng

200 bp

20 ng

100 bp

10 ng

Contents

1 vial EZ Load Precision Molecular Mass
Standard, 500 µl supplied in 5% glycerol, 15 mM
Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol
blue, 0.04% xylene cyanole FF.

Concentration

50 µg/ml

Determination

The concentration values of the EZ Load

of concentration

Precision Molecular Mass Standard fragments
are determined by UV absorbance at 260 nm
wavelength using the conversion factor:
1 . 0 0 A

2 6 0

unit = 50 µg/ml DNA. Fragment

concentrations are accurate to within ± 1%.

Please note

To maintain accurate fragment concentrations, it
is imperative to spin down any condensate and
mix contents thoroughly before opening tubes
and withdrawing aliquots.

1 2

1,000 bp

700 bp

500 bp

200 bp

100 bp

4006137b.qxd 09/16/1999 3:22 PM Page 12

This manual is related to the following products: