Bio-Rad Molecular Mass Rulers - Manual (Page 5)

Fig. 3. 5 µl of the EZ Load 100 bp PCR molecular ruler was loaded onto a
0.8% Molecular Biology Certified agarose gel. The gel was run at 140 V for
2.5 hours in 1x TBE buffer. The gel was stained in EtBr for 15 minutes and
destained in dH
2
O for 30 minutes.
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EZ Load 100 bp PCR Molecular Ruler
Catalog Number 170-8353
Use Load 5 µl per lane. This loading translates into
400 ng of DNA per lane. Adjustments may be
made to the loading volume for different well sizes
and desired band intensity. The EZ Load 100 bp
PCR molecular ruler can be resolved in 0.8–2%
agarose gels and polyacrylamide gels of 5%.
Contents 1 vial EZ Load 100 bp PCR molecular ruler,
500 µl supplied in 5% glycerol, 15 mM Tris
pH 8.0, 1.5 mM EDTA, 0.04% bromophenol
blue, 0.04% xylene cyanole FF.
Concentration 80 µg/ml
Size 30 bands: 100–3,000 bp in exact 100 bp
increments. Two visually distinct reference bands
at 1 kb and 3 kb contain three times the
concentration of material found in the other bands.
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3,000 bp
1,000 bp
1 0 0 b p
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