Bio-Rad Model 111 Mini IEF Cell - Manual (Page 3)

Table of Contents
Section 1 General Information................................................................................1
1.1 Introduction ...........................................................................................................1
1.2 Specifications.........................................................................................................1
1.3 Safety .....................................................................................................................2
1.4 Model 111 Mini IEF Cell Components .................................................................3
1.5 Capillary Thin Layer Gel Casting Tray .................................................................3
Section 2 Introduction to Isoelectric Focusing.......................................................4
2.1 The Electrofocusing Principle ...............................................................................4
2.2 Carrier Ampholytes ...............................................................................................4
2.3 Choice of Support Matrix ......................................................................................5
Section 3 Polyacrylamide Gel Isoelectric Focusing ...............................................5
3.1 Considerations in Matrix Preparation....................................................................5
3.2 Stock Solutions for Polyacrylamide IEF Gels.......................................................6
3.3 Reagents for Polyacrylamide Electrofocusing Gels ..............................................6
3.4 Use of Gel Support Film for Polyacrylamide........................................................7
3.5 Casting Polyacrylamide Gels ................................................................................8
3.6 Sample Preparation................................................................................................9
3.7 Sample Application .............................................................................................10
3.8 Position of Application........................................................................................10
Section 4 Running The Gel....................................................................................10
4.1 Set Up Proceedure ...............................................................................................10
4.2 Run Conditions ....................................................................................................11
Section 5 Sample Detection and Gel Storage .......................................................12
5.1 Removing the Gel................................................................................................12
5.2 Band Detection ....................................................................................................12
5.3 Destaining............................................................................................................13
5.4 Other Detection Methods ....................................................................................13
5.5 Gel Drying and Preservation ..............................................................................13
Section 6 Agarose Gel Electrofocusing .................................................................14
6.1 Introduction .........................................................................................................14
6.2 Preparing Agarose Gels .......................................................................................14
6.3 Sample Preparation..............................................................................................16
6.4 Sample Application .............................................................................................16
6.5 Position of Application........................................................................................16
6.6 Set Up Procedure .................................................................................................17
6.7 Run Conditions ....................................................................................................17
6.8 Sample Detection.................................................................................................18
Section 7 Troubleshooting .....................................................................................19
7.1 General Troubleshooting .....................................................................................19
7.2 Casting Troubleshooting, Polyacrylamide Gels ..................................................20
7.3 Casting Troubleshooting, Agarose Gels ..............................................................21