Bio-Rad ReadyPrep™ 2-D Starter Kit - Manual (Page 25)

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4. - The first line displays the step number, slope and voltage
- Select HRS:MIN (default)
- Enter time of 20 min.
- Press NEXT
S 01 250 V
HRS:MIN vhours >
ENTER TIME: 00:20 >
NEXT >
5. - Select ADD (default)
- Enter Step 2
- Press NEXT
ADD inset delete >
ENTER STEP #
2 >
NEXT >
METHOD COMPLETE >
6. - Enter step 2 final voltage
7 cm: 4,000 V; 11 cm: 8,000 V; 17 cm: 10,000 V)
- Select LINEAR ramp. Use key next to arrow, >, to move
the brackets, [ ] ,around the selection
- Press NEXT
S 02 xxxx V >
SELECT VOLTAGE SLOPE
LINEAR
[
д д]
>
NEXT >
7. - The first line displays the step number, slope and voltage
- Select HRS:MIN (default)
- Enter time
(7 cm: 2:00; 11 cm: 2:30; 17 cm 2:30)
S 02 xxxx V >
HRS:MIN vhours >
ENTER TIME: xx:xx >
NEXT >
8. - Select ADD (default)
- Enter step 3
- Press NEXT
ADD Inset delete >
ENTER STEP #
3 >
NEXT >
METHOD COMPLETE >
9. - Enter step 3 final voltage
(7 cm: 4,000 V; 11 cm: 8,000 V; 17 cm: 10,000 V)
- Select RAPID ramp. Use key next to arrow, >, to move
the brackets, [ ] ,around the selection
- Press NEXT
S 03 xxxx V >
SELECT VOLTAGE SLOPE
RAPID
[ ] >
NEXT >
- The first line displays the step number, slope and
voltage
- Select VHOURS (default)
- Enter time (volt hours)
(7 cm: 10,000; 11 cm: 20,000; 17 cm 40,000)
- Press NEXT
S 03 xxxx V >
HRS:MIN VHOURS
ENTER TIME: xxxxx >
NEXT >
10.