Bio-Rad ReadyPrep™ 2-D Starter Kit - Manual (Page 17)

3. Remove an IPG strip from the disposable rehydration/equilibration tray and dip briefly into
the graduated cylinder containing the 1X Tris/glycine/SDS running buffer, as shown in
Figure 10. Lay the strip gel side up and onto the back plate of the SDS-PAGE gel above
the IPG well (Figure 11). Repeat this process for any remaining IPG strips.
4. Take the first SDS-PAGE gel with the IPG strip resting on the back plate and hold or stand
vertically with the short plate facing towards you as in Figure 12. Use a pasteur pipette or
a Disposable Plastic Transfer Pipet (Bio-Rad catalog # 223-9522) and pipet overlay
agarose solution into the IPG well of the gel.
15
Fig. 11. Placing the ReadyStrip gel side up on the back plate of the SDS-PAGE gel.
Fig. 12. SDS-PAGE gel with ReadyStrip held vertically in rack. Pipetting overlay agarose into the IPG well.