Bio-Rad Trans-Blot® Cell - Manual (Page 8)

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4. Close the cassette firmly, being careful not to move the gel and filter paper sandwich.
Lock the cassette closed with the white latch.
5. Place the cassette in tank. Repeat for any other cassettes you may want to run. Completely
fill the tank with buffer.
6. If running high intensity or high voltage with plate electrodes, add the super cooling coil
in any available slot.
Note: For high intensity runs (4 cm inter-electrode distance) only one cassette can be
placed in the tank. The Super Cooling Coil must be used. It is placed outside the electrodes.
See Section 2.2, high intensity field option. Two cassettes can be run with the Super
Cooling Coil in the standard field with the electrodes 8 cm apart, and three cassettes can
be run at low voltage without the super cooling coil.