Bio-Rad Trans-Blot® Cell - Manual (Page 10)

2.2 High Intensity Field Option
The electrode cards of the Trans-Blot cell can be moved closer together to allow higher
field strengths (V/cm), and thus more efficient, more rapid transfer. To decrease the inter-
electrode distance, the anode electrode card is moved to the center gel holder position.
1. Remove the screws at the top corners of the anode (red) electrode card.
2. Set up the gel holder with the gel, pads, and membrane following the standard Trans-
Blot cell operation instructions outlined in Section 2.1. Insert the sandwich into the gel
holder slot closest to the cathode (black) electrode panel.
3. Pull the anode card out of the cell, and move it into the middle gel holder slot. The gel
holder will keep the electrode card firmly positioned in the slot.
4. Completely fill the buffer tank to the bottom of the red electrode disc.
5. Place the Super Cooling Coil into the free gel holder slot and place a stir bar in the bot-
tom of the tank. The Super Cooling Coil must be used for adequate heat dissipation of gen-
erated in high-intensity transfer. Set stirring speed as high as possible.
6. Remove the white plug from the electrode cable hole in the Trans-Blot cell lid. Unscrew
the two red screws anchoring the red power cable to the lid. Loosen the screws 4 turns.
Move the cable to its alternative position, aligning shallow holes in the cable with the
anchor screws, and retighten all screws.
7. Begin electrophoretic blotting.
The anode electrode is removed from the standard position and placed 4 cm apart from the
cathode. The cable is also moved to line up with electrodes.
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