1 preparation for blotting – Bio-Rad Trans-Blot® Cell User Manual
Page 7
Section 2
Trans-Blot Cell Assembly
2.1 Preparation for Blotting
1. Prepare the transfer buffer. (See Section 3.3 for buffer formulation. Using buffer chilled to
4 °C will improve heat dissipation.)
2. Cut the membrane and the filter paper to the dimensions of the gel. Always wear gloves
when handling membranes to prevent contamination. Equilibrate the gel and soak the
membrane, filter paper, and fiber pads in transfer buffer (15 min—1 hour depending on
gel thickness).
3. Prepare the gel sandwich.
Place the cassette, with the gray side down, on a clean surface.
Place one pre-wetted fiber pad on the gray side of the cassette.
Place a sheet of filter paper on the fiber pad.
Place the equilibrated gel on the filter paper.*
Place the pre-wetted membrane on the gel.*
Complete the sandwich by adding a piece of filter paper on the membrane.*
* Removing any air bubbles which may have formed is very important for good
results. Use a glass tube to gently roll air bubbles out.
Add the last fiber pad.
4
Fiber pad
Filter paper
Membrane
Gel
Filter paper
Fiber pad
Cathode (-) side
Anode (+) side
- TransFectin™ Lipid Reagent (2 pages)
- Gene Pulser MXcell™ Electroporation System (19 pages)
- Gene Pulser MXcell™ Electroporation System (66 pages)
- Gene Pulser Xcell™ Electroporation Systems (83 pages)
- Gene Pulser® Electroporation Buffer (2 pages)
- MicroPulser™ Electroporator (31 pages)
- Helios® Gene Gun System (52 pages)
- PDS-1000 / He™ and Hepta™ Systems (51 pages)
- TGX™ FastCast™ Acrylamide Solutions (2 pages)
- Criterion™ TGX Stain-Free™ Precast Gels (16 pages)
- Image Lab™ Software (212 pages)
- Gel Doc™ EZ System (22 pages)
- Criterion Stain Free™ Tris-HCl Gels (96 pages)
- Mini-PROTEAN® TGX™ Precast Gels (52 pages)
- ChemiDoc™ XRS+ System (42 pages)
- ChemiDoc™ XRS+ System (4 pages)
- ChemiDoc™ XRS+ System (50 pages)
- GS-800™ Calibrated Densitometer (444 pages)
- ChemiDoc™ MP System (8 pages)
- Image Lab™ Software (236 pages)
- Criterion™ TGX™ Precast Gels (60 pages)
- Criterion™ Cell (13 pages)
- Image Lab™ Software (260 pages)
- Criterion™ XT Bis-Tris Precast Gels (26 pages)
- 2-D Electrophoresis Workflow (14 pages)
- Fluorescent Protein Stains (27 pages)
- 2-D Electrophoresis Workflow (22 pages)
- 2-D Electrophoresis Workflow (20 pages)
- PROTEAN® i12™ IEF System (4 pages)
- ReadyPrep™ 2-D Starter Kit (28 pages)
- EXQuest Spot Cutter (81 pages)
- Ready Gel® Zymogram Precast Gels (46 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (44 pages)
- Precision Plus Protein™ Prestained Standards (3 pages)
- Precision Plus Protein™ Unstained Standards (16 pages)
- Prestained SDS-PAGE Standards (3 pages)
- Unstained SDS-PAGE Standards (3 pages)
- Silver Stains (20 pages)
- Biotinylated Standards (3 pages)
- Biotinylated Standards (11 pages)
- IEF and 2-D Standards (3 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (20 pages)
- Mini-PROTEAN 3 Multi-Casting Chamber (10 pages)
- PROTEAN® Plus Hinged Spacer Plates and Combs (21 pages)
- Mini-PROTEAN® Tetra Handcast Systems (10 pages)