Bio-Rad Mini-PROTEAN® 3 Dodeca™ Cell - Manual (Page 7)

d. While gently squeezing the gel sandwiches or cassettes against the green gaskets, slide the
arms of the clamping frame over the gels, locking them into place. This forms the clamp
assembly.
e. The arms of the clamping frame push the short plates up against the notch in the green
gasket, creating a leak-proof seal. At this point, the sample wells can be washed out with
running buffer, and sample can be loaded.
f. Place the Dodeca cell on a stir plate.
g. Lower each clamp assembly into one of the six slots in the tank.
h. Fill the tank with at least 2.5 liters of buffer (3.5 liters recommended) and drop a 38 mm
(1.5") stir bar into the buffer tank. Do not exceed the maximum fill line. Fill each clamp
assembly with 150 ml of running buffer (900 ml total for 6 clamp assemblies.)
i. Turn on the stir plate and make sure the stir bar is mixing effectively.
5