Bio-Rad Mini-PROTEAN® 3 Dodeca™ Cell - Manual (Page 4)

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Important: This Bio-Rad product is designed and certified to meet *IEC61010-1 and
EN61010-1 safety standards. Certified products are safe to use when operated in accordance
with the instruction manual. This instrument should not be modified or altered in any way.
Alteration of this instrument will
• Void the warranty
• Void the IEC61010-1 and EN61010-1 certifications, and
• Create a potential safety hazard
Bio-Rad is not responsible for any injury or damage caused by use of this instrument for
purposes other than those for which it is intended or by modifications of the instrument not
performed by Bio-Rad or an authorized agent.
*IEC61010-1 and EN61010-1 are internationally accepted electrical safety standards for laboratory instruments.
1.3 Components
To get the best performance from your Mini-PROTEAN 3 Dodeca cell, familiarize
yourself with the components by assembling and disassembling the cell before using it.
Buffer tank and lid The buffer tank and lid combine to fully enclose
the inner chamber during electrophoresis. The
lid cannot be removed without disrupting the
electrical circuit.
Cooling coil The integrated cooling coil chills the lower
buffer chamber. The cooling core must be
connected to a refrigerated circulator.
Electrode assembly The lower buffer chamber contains the anode.
The lid of the Dodeca cell houses the cathode.
Drain port The drain port allows buffer to be removed
from the tank. The drain line attaches via a
quick connect fitting; this assembly is
provided with the cell.
Clamping frame 1 The clamping frame holds two gel sandwiches
in place. It houses the notched gasket.
Buffer dam The molded, one piece buffer dam is used
when running only one gel in a clamping
frame.
Gel releaser The gel releaser is a small green tool that
loosens the two glass plates following
electrophoresis.
Gel sandwich A spacer plate and a short plate with a
polymerized gel form a gel sandwich.
Gel cassette A Ready Gel precast gel or a handcast gel in
an empty Ready Gel cassette is referred to as a
gel cassette.
1 U.S. Patent 6,436,262.