Bio-Rad MicroPulser™ Electroporator - Manual (Page 6)

2
Warning: The MicroPulser generates, uses, and radiates radio frequency energy. If it is not
used in accordance with the instructions given in this manual, it may cause interference with
radio communications. The MicroPulser has been tested and found to comply with the limits
for Class A computing devices (pursuant to Subpart J of Part 15 of FCC Rules) which provide
reasonable protection against such interference when operated in a commercial environment.
Operation of this equipment in a residential area is likely to cause interference. In this case the
user will be required, at their own expense, to take whatever measure may be required to correct
the interference.
Section 2
Introduction
2.1 Overview of Electroporation Theory
The MicroPulser system is used for the electroporation of bacteria, yeast, and other
microorganisms where a high voltage electrical pulse is applied to a sample suspended in a
small volume of high resistance media. The system consists of a pulse generator module, a
shocking chamber, and a cuvette with incorporated electrodes (Figure 1). The sample is placed
between the electrodes in the cuvette. The MicroPulser module contains a capacitor, which is
charged to a high voltage; the module then discharges the current in the capacitor into the
sample in the cuvette.
Fig. 1. MicroPulser consisting of Pulse Generator Module, Shocking Chamber and Cuvette.