Bio-Rad MicroPulser™ Electroporator User Manual

Page 13

Fig. 5. MicroPulser control panel.

Section 4
MicroPulser Operating Instructions

Refer to Figure 1 for a view of the components of the MicroPulser system and to Figure 5

for a definition of the buttons and LEDs.

4.1 Setting Up The MicroPulser System

1. Connect the black power cord to the rear panel of the MicroPulser Pulse Generator

Module. Plug the cord into a wall outlet or power strip.

2. Pull down the fold-down foot on the underside of the MicroPulser. Insert this foot into the

track on the base of the shocking chamber. Insert the shocking chamber slide into the
shocking chamber.

3. Connect the leads from the shocking chamber to the output jacks on the front panel of the

MicroPulser; polarity is not important to the electroporation process.

4. Turn on the apparatus using the power switch on the right rear panel. The light emitting

diode (LED) display should illuminate and read “Ec1” and the LED next to the Bacteria
Settings should be illuminated.

4.2 Operation of the MicroPulser

1. Selecting Pre-Programmed Settings

The MicroPulser is pre-programmed with settings for electroporation of a number of

commonly used organisms. Included under the Bacteria Settings program are the following:

Mnemonic

Organism

Parameters

Voltage

Number of

Time constant

(kV)

pulses

(msec)

Ec1

E. coli (0.1 cm cuvette)

1.8

Ec2

E. coli (0.2 cm cuvette)

2.5