Bio-Rad MicroPulser™ Electroporator User Manual

Page 14

StA

S. aureus (0,2 cm cuvette)

1.8

2.5

Agr

A. tumefaciens (0.1 cm cuvette)

2.2

Ec3

E. coli (0.2 cm cuvette)

3.0

Included under the Fungi Settings program are the following:

Mnemonic

Organism

Parameters

Voltage

Number of

Time constant

(kV)

pulses

(msec)

Sc2

S. cerevisiae (0.2 cm cuvette)

1.5

Sc4

S. cerevisiae (0.2 cm cuvette)

3.0

ShS

S. pombe (0.2 cm cuvette)

2.0

dic

D. discoideum (0.4 cm cuvette)

1.0

Pic

P. pastoris (0.2 cm cuvette)

2.0

Pressing the "Settings" button cycles the Settings LED between "Bacteria", "Fungi", and

"Manual". When the LED next to Fungi is lit, the mnemonic for the fungi programs are
displayed. Pressing the "Raise" and "Lower" buttons to the left of the display LED cycles
between the different fungi programs. When the mnemonic is displayed, the parameters
associated with the mnemonic are automatically selected.

To change from one bacteria setting to another, while the Settings LED next to Bacteria

is lit, press the "Raise" and "Lower" buttons to the left of the display LED to cycle between
the different programs. When the mnemonic is displayed, the parameters associated with the
mnemonic are automatically selected.

While a program mnemonic is displayed for either a Bacteria or Fungi Setting,

simultaneously pressing both the "Raise" and "Lower" buttons shows the program
parameters selected on the display LED. The display LED first shows the voltage value, then
displays a "t" followed by the time in msec, then, if time and multiple pulses are associated
with a program, display "P" followed by "2", indicating that two successive pulses are given.
If no "t" is given the pulse is not truncated, and if no "P" is given there is a single pulse.

2. Using The Micropulser in Manual Mode

A. To change the voltage.

Press the "Settings" button to illuminate the LED next to "Manual". The display LED
now shows the voltage (in kV). Pressing the "Raise" and "Lower" buttons to the left of the
display LED allows selection of the desired voltage in the range from 0.20 kV to 3.00 kV.
If the instrument was just turned on, the display LED shows "0.00"

B. To truncate the pulse.

Press both "Raise" and "Lower" buttons simultaneously while the "Manual" LED is
illuminated. The display LED now shows “t—” and indicates the pulse time that has been
selected for the pulse. The default setting when the power is turned on is the standard
exponential decay pulse, or no pulse truncation, which is indicated by the two dashes.
Releasing only the "Lower" button results in the display LED showing the time of the
truncated pulse in milliseconds. The display LED initially changes to "t1.0" and rises in
0.1 msec increments to "t4.0". This permits truncating the exponential decay pulse between
1–4 msec. Simultaneously pressing both "Raise" and "Lower" buttons, then releasing
only the "Raise" button, results in lowering the indicated truncation time.