Bio-Rad SingleShot™ Cell Lysis RT-qPCR Kits - Manual (Page 7)

© 2014 Bio-Rad Laboratories, Inc. 10042474
SingleShot ™ SYBR ® Green Kit
Amplification
Real-Time PCR System
Setting/ Scan
Mode
Polymerase Activation and
DNA Denaturation
Denaturation
at 95°C, sec
Annealing/Extension and Plate Read
at 60°C, sec* Cycles Melt Curve Analysis
Bio-Rad ® CFX96 ™ ,
CFX384 ™ , CFX96 Touch ™ ,
CFX96 Touch Deep Well,
CFX384 Touch
™ ,
CFX Connect ™
SYBR ® only
30 sec at
98°C for cDNA
5–15
15–30
35–40
65–98°C
0.5°C increments
at 2–5 sec/step
(or use instrument
default setting)
Bio-Rad ® iQ ™ 5,
MiniOpticon ™ , Chromo4 ™ ,
MyiQ ™
Standard 15–30
Applied Biosystems 7500
and 7900 HT, QuantStudio
Standard 15–30
Roche LightCycler 96 or 480
Fast 10–30
Standard 60
QIAGEN Rotor-Gene and
Stratagene Mx series
Fast 15–30