Appendix a – Bio-Rad SingleShot™ Cell Lysis RT-qPCR Kits User Manual
Page 12
© 2014 Bio-Rad Laboratories, Inc.
10042474
SingleShot
™
SYBR
®
Green Kit
Appendix A
Table 1. Setting up SingleShot cell lysis reactions.
Step
Guidelines
Preparing the
cell culture
Number of wells
384
96
48
24
12
6
Cell numbers per
well at harvest
10–
5 x 10
4
10–
1 x 10
5
10–
2 x 10
5
10–
4 x 10
5
10–
8 x 10
5
10–
1 x 10
6
Washing the
cells with PBS
Volume of PBS
per well, µl
30
125
250
500
1,000
2,000
Preparing the
SingleShot
cell lysis
master mix
SingleShot Cell
Lysis Buffer, µl
12
48
96
192
384
768
Proteinase K, µl
0.25
1
2
4
8
16
DNase, µl
0.25
1
2
4
8
16
Total volume
per well, µl
12.5
50
100
200
400
800