LaMotte EZ 3-0034 - Manual (Page 5)

Dis posal
Any materials containing living or viable microbes should be disinfected or sterilized
before being discarded. Before disposal in normal trash, treat the test kit components
in one of the following ways:
• Pour one tablespoon of undiluted household bleach on the dish and one
tablespoon in the Coliscan Easygel bottle. Wait 10 minutes. Place the dish and
bottle in a waterproof bag. Discard in trash.
• Place the dish and uncapped Coliscan Easygel bottle in a large pan. Cover
with water. Boil for 45 minutes. Discard in trash. (Warning: May cause
unpleasant odors.)
• Place the dish and uncapped Coliscan Easygel bottle in a sealed ovenproof
bag and heat in a 300°F oven for 45 minutes. Discard in trash. (Warning: May
cause unpleasant odors.)
• Place dish and uncapped Coliscan Easygel bottle in a pressure cooker and
cook at 15 pounds for 15 minutes. Discard in trash. (Warning: May cause
unpleasant odors.)
Ex ten sions
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Alternative Method – Streak Plate (Swabbing)
1. Lay Petri dish on a level surface with the lid side up. Remove the lid.
2. Remove the cap from the Coliscan Easygel bottle. Do not touch the rim or inside
of the bottle or the Petri dish.
3. Pour all the liquid Coliscan Easygel into the dish bottom. Replace the lid. Gently
swirl to cover the bottom.
4. Wait 45 minutes until the Coliscan Easygel has solidified. Pour dishes a day in
advance for a firmer surface area.
5. Inoculate the dish.
How clean are your fingers? Divide a poured Coliscan Easygel dish in half by
turning the dish over and drawing a line across the bottom with a wax pencil or
marker. Remove the lid and touch several fingers to the surface on one half. Wash
your hands thoroughly with soap and water, dry on a clean paper towel, and touch
the washed fingers to the other half. Label and incubate. Check at 24, 48, and 72
hours for growth. Did you get less growth after washing? Why or why not?
How clean is your doorknob or table? Pour a dish of Coliscan Easygel a day in
advance. Save the bottle with the Coliscan Easygel residue. Use a sterile swab, which
has been moistened with the Coliscan Easygel residue from the empty bottle, to wipe
a doorknob, handle or tabletop. Rotate the swab so that the entire surface has made
contact. Remove the lid of the poured dish. Start on one side of the dish and gently
wipe the swab against the surface of the gel. Rotate the swab while moving it in a zig
zag pattern. Wiping too hard will tear the gel. Put the lid back on the dish and
incubate. Check at 24, 48 and 72 hours for growth.
Are insects dirty?: Using a poured dish of Coliscan Easygel, place a live insect on
the gelled medium, and replace the lid. Remove the insect with clean forceps after it
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