LaMotte AG-104 Salt Water Aquarium - Manual (Page 4)

AMMONIA NITROGEN
Salicylate Method
QUAN TITY CON TENTS CODE
60 mL *Salicylate Ammonia #1 *3978LWT-H
30 mL *Salicylate Ammonia #2 *3979WT-G
30 mL Salicylate Ammonia #3 3982WT-G
2 Test Tubes, plastic, w/cap 0106
1 Octa-Slide Viewer 1100
1 Ammonia-Nitrogen Octa-Slide Bar,
0-2 ppm
3441
*WARNING: Reagents marked with a * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS
CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by email, phone
or fax.
Pro ce dure
1. Fill a test tube (0106) to the 5 mL line with the water sample.
2. Add 10 drops of Salicylate Ammonia #1 (3978LWT). Cap and mix.
3. Add 7 drops of *Salicylate Ammonia #2 (3979WT). Cap and mix.
Wait one minute.
4. Add 7 drops of Salicylate Ammonia #3 (3982). Cap and mix. Wait
20 minutes.
5. Insert Ammonia Nitrogen Octa-Slide Bar (3441) into the
Octa-Slide Viewer (1100). Insert test tube into Octa-Slide Viewer.
Match sample color to a color standard. Record as ppm Ammonia
Nitrogen (NH
3
_ N).
To express results as Unionized Ammonia (NH
3
):
ppm Unionized Ammonia (NH
3
) =
ppm Ammonia Nitrogen (NH
3
–N) x 1.2
To express results as Ionized Ammonia (NH
4
):
ppm Ionized Ammonia (NH
4
+ ) =
ppm Ammonia Nitrogen (NH
3
–N) x 1.3
Ammonia in water occurs in two forms: toxic unionized ammonia
(NH
3
) and the relatively non-toxic ionized form, ammonium ion
(NH
4
+ ). This test method measures both forms as ammonia nitrogen
(NH
4
+ –N) to give the total ammonia nitrogen concentration in water.
The actual proportion of each compound depends on temperature,
salinity, and pH. A greater concentration of unionized ammonia is
present when the pH value and salinity increase.
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