Metrohm Inline Dialysis - Manual (Page 25)

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4 Operation and maintenance
IC equipment: Dialysis
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4 Putting the dialysis system into operation
￭ Immerse the aspiration capillary for the acceptor solution in the
acceptor solution.
￭ Immerse the sample aspiration capillary in a rinsing solution (e.g.
ultrapure water).
￭ Start both peristaltic pumps in the software.
￭ Rinse the dialysis system with the acceptor solution and the rins-
ing solution for approx. 10 minutes and then switch off the peri-
staltic pumps again.
5 Calibrating the IC system
￭ Immerse the sample aspiration capillary in the standard solution.
￭ Start the determination in the software and wait until the stand-
ard chromatogram has been evaluated.
￭ Repeat the two steps for all standard solutions.
6 Determining the sample
￭ Immerse the sample aspiration capillary in the sample solution.
￭ Start the determination in the software and wait until the chroma-
togram has been evaluated.
7 Rinsing the dialysis system
￭ After the end of the measurements, immerse the sample aspira-
tion capillary in the rinsing solution.
￭ Switch on the peristaltic pump for the sample channel in the soft-
ware.
￭ Rinse the dialysis system with the acceptor solution and the rins-
ing solution for approx. 10 minutes and then switch off the peri-
staltic pumps again.
8 Taking the dialysis system out of operation
￭ If the dialysis cell is taken out of operation, then you have to rinse
the acceptor channel and the sample channel with ultrapure
water for approx. 10 minutes.
Then remove all four capillaries of the dialysis cell and close each
opening with a threaded stopper (6.2744.060).
￭ If the dialysis cell is taken out of operation for a longer period,
then you have to remove the dialysis membrane and clean the
dialysis cell (see Chapter 3.1, page 6).
￭ Release the contact pressure in order to relieve the pump tubings
(see the chapter "Peristaltic pump" in the manual for the Sample
Processor).