Metrohm Inline Dialysis - Manual (Page 24)

4.1 Operation
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IC equipment: Dialysis
2 Measuring the standard solution with dialysis
￭ Set the dialysis time in the software to 5 minutes.
￭ Immerse the sample aspiration capillary in the standard solution
used in step 1.
￭ Start a determination in the software and wait until the chroma-
togram has been evaluated.
3 Additional measurements
￭ Increase the dialysis time in the software by 1 minute each time
and start the determination.
￭ Repeat the measurements until the measured values are stable.
4 Determining the optimum dialysis time
￭ The recovery rates can be calculated from the result without dialy-
sis and the results with dialysis. You can determine the optimum
dialysis time by recording the recovery rate in relation to the dialy-
sis time.
4.1.2 Recommended procedure for the dialysis
For sample determination with dialysis, it is best to proceed according to
the following sequence:
Carrying out a dialysis
1 Preparing the acceptor solution
￭ To avoid disruptions caused by air bubbles in the acceptor flow,
degas the ultrapure water used as acceptor solution for at least
10 minutes.
2 Preparing the sample
￭ To avoid blockage of the sample channel, always centrifuge sam-
ples with a high content of suspended or solid particles in a tab-
letop centrifuge for 5 minutes at 10,000 rpm.
3 Putting the IC system into operation
￭ Switch on all required devices.
￭ Start the software and load the required IC system.
￭ Condition the IC system.