2 protecting the separation column, 3 storing the separation column, 4 regenerating the separation column – Metrohm 940 Professional IC Vario ONE/SeS/PP/HPG User Manual
Page 110: Protecting the separation column, Storing the separation column, Regenerating the separation column
5.17 Separation column
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100
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940 Professional IC Vario ONE/SeS/PP/HPG (2.940.1540)
representative) or on the Internet at
Chromatography product area. You can find information on special IC
applications in the corresponding "Application Bulletins" or "Applica-
tion Notes
in the Applications area or free of charge upon
request from your responsible Metrohm representative.
5.17.2
Protecting the separation column
We recommend using the following protective measures so that the sepa-
ration column retains its separating efficiency for as long as possible:
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Microfilter both the sample and the eluent (0.45 µm filter) and aspirate
the eluent via the aspiration filter (6.2821.090) as well.
■
Always use a guard column. Information regarding which guard col-
umn is suitable for your separation column can be found in the
Metrohm IC Column Program (which is available from your
Metrohm representative), the leaflet provided along with your separa-
tion column, the product information about the separation column at
obtained directly from your representative.
■
Always use the pulsation absorber.
5.17.3
Storing the separation column
Always store separation columns you do not need in a sealed and filled
state according to the column manufacturer's specifications.
5.17.4
Regenerating the separation column
The separation column can be regenerated according to the column man-
ufacturer's specifications if the separation characteristics of the column
have deteriorated. You can find information on regenerating separation
columns available from Metrohm on the leaflet provided with every col-
umn.
NOTE
Regeneration is intended as a last resort. It should not be carried out
regularly.