UVP iBox Anesthesia System - Manual (Page 13)

iBox Anesthesia System 13
Animals are too deeply anesthetized:

Usually in this case the isoflurane/sevoflurane concentration on the vaporizer is too high. If
an animal is observed to be in danger of dying, reduce the isoflurane/sevoflurane
concentration to zero and let pure oxygen flow until the animal returns to a normal state of
anesthesia. Then, turn the isoflurane concentration to a lower value than previously used.
Breathing should be even and not appear labored. Limbs should appear pink, not blue.

If an animal is anesthetized on the breathing device and oxygen supply is suddenly cut off
or drastically reduced, the animal may suffer from a lack of oxygen. Ensure that the
oxygen tank contains adequate oxygen for the length of the procedure and that the gas
supply to the breather is not obstructed in any way.

During hours-long procedures, anesthetic can build up in the animal. It may be necessary
to reduce isoflurane concentration over time.

During long procedures, animals may become hypothermic. Ensure that the iBox warming
plate is on and set to the appropriate temperature.

If a particular species or strain of animal is consistently dying on the anesthesia machine,
an adjustment must need to be made to the procedure for that species to reflect a lower
working concentration of isoflurane/sevoflurane.
The vaporizer inlet/outlet cap popped off during operation:

The inlet/outlet caps connecting to the vaporizer are designed to pop off when pressure
builds up in the system. This occurs when gas entering the system has an insufficient
outlet.

At least one of the Chamber Control or Breather Control valves must be turned to the On
position at all times while the system is in use.

Ensure that the quick disconnect fittings leading to the breather and chamber are
completely mated. The collar on the female metal fittings must be completely pushed
forward for complete mating to occur. Plastic fittings will give an audible ‘click’ when
properly mated.

Ensure that none of the hosing is kinked.
I can smell isoflurane/sevoflurane outside the iBox:

The charcoal filter canisters may be saturated with isoflurane. Weigh the filters and
discard any that have increased in weight by 100 grams since new. Replace any filters
that have been used but were never weighed.

Ensure that the Chamber Control valve is turned to the Off position and that the Breather
Control valve is turned to the Breather On position immediately prior to removing the
animals from the chamber and while the chamber is not in use.
I can smell isoflurane/sevoflurane inside the iBox:

Ensure that any nosecones not in use are plugged with the included black threaded
nosecone plugs.

Ensure that there is a tight seal around the animal’s nose(s) on the nosecone(s).

Ensure that all of the hosing to and from the breather is connected and that the exhaust
hose coming from the back of the iBox is connected.

The hoses connecting the breather may be reversed. If this occurs, gas will exhaust
directly into the iBox. Refer to the installation instructions in this manual on how to
properly connect these hoses.