C.B.S. Scientific MGV-216-33 - Manual (Page 13)

C.B.S. œ Scientific Double & Triple Wide Mini-Vertical 13
3.6 Maximum Well/Comb Volumes
NOTE: To calculate sample well volume expressed in millimeters (mm) of height,
divide maximum volume by tooth depth.
Rectangle Combs for Double Wide Mini-Vertical
# of wells
Tooth width/mm 0.75mm thickness
volume per tooth
microliters ( μl)
1.0mm thickness
vloume per tooth
microliters ( μl)
1.5mm thickness
volume per tooth
microliters ( μl)
12 11 82.5 110 165
14 9.1 68.3 91 136.50
16 7.7 57.75 77 115.5
17 6.7 50.25 67 100.5
20 5.66 42.45 56.6 84.9
28 4 30 40 60
32 3.4 25.5 34 51
35 2.95 22.13 29.5 44.25
Overall length of comb: 15.87cm
Tooth depth: 10mm
Spacing between teeth: 12-20 wells = 2.3mm 28-35 wells = 1.5mm
Rectangle Combs for Triple Wide Mini-Vertical
# of wells
Tooth width/mm 0.75mm thickness
volume per tooth
microliters ( μl)
1.0mm thickness
vloume per tooth
microliters ( μl)
1.5mm thickness
volume per tooth
microliters ( μl)
31 5.8mm 43.50 58 87
34 6.05mm 45.38 60.50 90.75
50 4.05mm 30.38 40.50 60.75
60 3.18mm 28.35 31.80 47.70
63 2.95mm 22.13 29.50 44.25
102 2mm 15 20 30
Overall length of comb: 28.4cm
Tooth depth: 10mm
Spacing between teeth: 31-34 wells = 2.4mm 50-63 wells = 1.5mm
SECTION 4
Running Conditions
4.1 Recommended Power
Precise electrophoresis conditions will vary according to the number and type of gels
used, buffer conditions employed, power input, and the general goal of the experiment.
Refer to the reference section for in-depth discussions on practical and theoretical
approaches to protein gel electrophoresis.
Using standard SDS-PAGE buffer systems apply 1-10 VDC/cm of gel. If running two
gels in the dual units, keep the volts the same but double the mA. It is also true that if the
thickness of the gel increases, increase the mA proportionally.
At constant voltage, the proteins will migrate at a constant rate during electrophoresis
with adequate heating appropriate for denaturing gels. Increasing the voltage/mA (for a
single gel thickness and percentage) will speed mobility but increase the risk of
overheating.
The sample migration rate can be increased by raising the input power. This can be
done in the dual systems, which employ ‘active’ temperature control. The joule heating
generated by the higher power is offset by the cooling effect of the water between the
gels. Exact conditions should be determined empirically but should be at least in the
20% range.