C.B.S. Scientific MGV-216-33 - Manual (Page 10)

C.B.S. œ Scientific Double & Triple Wide Mini-Vertical 10
5. Lift the assembly and stand it on the base of the clamps. For leveling, push
glass plate assembly down until it stops against clamp body. Clamp the sides of
the assembly with additional casting clamps on either side. As each clamp is
attached, be sure the gasket is aligned between the plates forming a seal.
6. Apply PAGE or agarose solution to gel plate sandwich using a syringe or pipette.
Pour the gel from the center of the glass plates. If using a stacking gel, pour
desired height of running gel, then overlay a small amount of dH
2
O or 0.1% SDS
solution to top of gel.
7. After polymerization, rinse with buffer, add stacking gel solution and insert comb.
For regular unit percentage gels, add polyacrylamide solution to correct height,
and insert comb. Allow gel to set, usually 20 minutes. Extra gel solution in
pipette or syringe can be monitored to test polymerization of gel mix.
8. Disassembly. Hold the clamped plate assembly with one hand. Remove the
gasket by starting at one top end and pulling up and out on the gasket until it
releases from the plate. When each clamp is reached DO NOT remove it,
instead feed the gasket down through the clamp body and repeat pulling up and
out. Continue feeding until the gasket is fully detached. If gel, is not to be used
immediately, wrap entire plate sandwich with plastic wrap tightly to seal, overlay
with H
2
O and store upright at 4
°C for up to a month.