C.B.S. Scientific QNX-700 - Manual (Page 9)

9 www.cbsscientific.com
SECTION 3
Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels
3.1 Preparing the Electrophoresis Unit
1. Power leads are shipped separately and
must be attached before using your
Quadra. Thread the power lead ends into
the lid receptacles and rotate in clockwise
direction to hand tighten.
2. Place unit in authorized work area. Remove
safety cover from the assembled unit by
simultaneously pressing down on white push
pins while lifting up on blue safety cover as
shown in figure 1.
Do not remove safety cover by pulling up
on leads!
3. Remove white core from lower reservoir
by grasping core with one hand and lifting
directly up as shown in figure 2. Remove
second core in same manner.
4. Open doors on the core assemblies by
pulling up on the white latches, as shown in
figure 3.
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5. Slide pre-cast gel cassette or plate set(s) into
the core assemblies with the notched plate
facing in towards the upper buffer reservoir
as shown in figure 4. If using a pre-cast gel
stored at 4°C, allow to warm to room tem-
perature. If pouring your own gels please see
Alternate Protocol (Section 4) on pages 12-
14 describing gel casting using Gel Wrap™.
6. If running one gel, slide white plastic adaptor
plate into the side without the gel. If running
a Lonza 10cm x 9cm gel casssette place
clear shim as shown in figure 5.
7. Close doors and relatch by pressing down
on the white latches so that the assembly
looks like that shown in figure 6.
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