C.B.S. Scientific QNX-700 - Manual (Page 18)

18
Quadra Instructions 9-30-13
6.2
Electro-Blotting
6.3 References
1. Hames, B.D. (ed.) (1998). Gel Electrophoresis of Proteins. A Practical Approach.
3 rd edn. Oxford University Press, Oxford. Ch. 1,3.
2. Sambrook, J., Fritsch, Russell, D. (2001). Molecular Cloning. A Laboratory
Manual. 3 rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New
York. A8.40-A8.55
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith,
J.A., Struhl, K. (ed) (1993). Current Protocols in Molecular Biology. Vol. 2,
Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., Ch. 10.
As a general recommendation, equilibrate gels (after running) with the diluted transfer
buffer for 5 to 10 minutes before transfer.
Blotting Buffer
ClearPAGE™ Transfer Buffer 10X/20X
cat. # FB82500
(see Section 6.4)
100ml
(1:10 dilution)
Methanol 200ml
Ultrapure Water 720ml
Typical Blotting conditions for Quadra
Power Supply Set-
ting
200V constant
Blot time 1.5 - 2.0 hours with stirring
Expected current 180mA / 1 gel
220mA / 2 gels
300mA / 4 gels