C.B.S. Scientific QNX-700 - Manual (Page 10)

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Quadra Instructions 9-30-13
3.2 Running the Gel
1. Place stirring bar into bottom of reservoir in
stirring corral.
2. Place core assemblies into lower reservoir.
The anode (red) and cathode (black) elec-
trodes are color-coded on both the core/
cassette assembly and lower reservoir. See
figure 7. Ensure the red dot on the cassette
assembly is on the same side as the red re-
ceptacle on the lower reservoir. Fill both core
upper reservoir with freshly prepared buffer
(~ 400mls).
If any buffer is spilled into banana jack
receptacles (outlined in yellow boxes in
figure 7) in lower reservoir, dry completely
using compressed air! Failure to do this
will result in accelerated banana jack cor-
rosion. Quadra should look like figure 8.
3. Pour only enough freshly prepared buffer into
lower chamber so that the final buffer level
is just below bottom of sample wells. Using
a pipette or syringe, thoroughly flush out the
wells in the glass plate sandwich with buffer.
Load samples. If outer lanes do not contain
sample, it is recommended that you run
standards and/or fill outer lanes with loading
buffer to reduce smiling and wrap-around
effects.
4. Attach safety cover and turn on mag-
netic stirrer. If you have the cooled unit, cat.
#QNC-700, precool buffers and attach exter-
nal cooler (set to the desired temperature) to
the tubing adaptors so that the inlet is on the
bottom and the outlet is on top as shown in
figure 9. The Quadra is now ready to attach
to the power supply.
5. Connect the leads to the power supply,
matching the color-coded red to red and
black to black as shown in figure 10.
See
Section 6.1 for recommended power con-
ditions. Begin separation by electrophoresis.
matching
colors
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9
stirring
bar in
stirring
corral
outlet
inlet
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