Luminex MasterPlex QT Tutorial Manual - Manual (Page 44)

http://www.miraibio.com 44 Master Plex QT
b. Fold Change – If the With normalization check box is ticked, it will perform
a normalization step before the fold change calculation between the treatment and
control groups.
You have 3 options when normalizing:
1. Normalize by a single house keeping gene.
2. Normalize by the geometric mean of all the housekeeping genes
selected in Step 1 if 2 or more genes were selected.
3. Normalized by a Constant value.
c. Normalization – This performs the data normalization step only using one of
the 3 options discussed above.