Luminex MasterPlex QT Tutorial Manual - Manual (Page 12)

http://www.miraibio.com 12 Master Plex QT
lowest point on the standard curve, we can infer that the concentration for that analyte is
less than the concentration of the lowest point on the standard curve for that analyte. So,
in the example above, we cannot use the model to calculate an estimated concentration,
but we can infer that the concentration is less than 26.47 pg/mL. There is a similar upper
limit on the 4-parameter logistic and 5-parameter logistic curve models, with an
analogous “>” nomenclature when the MFI value is above the upper limit of the curve
model, but we can infer the concentration is above the highest point on the standard curve
for that analyte.
For many users, it is enough to know that we can infer “the concentration is below the
lowest point on the standard curve” or “the concentration is above the highest point on
the standard curve” when we measure a data point outside the range of the model curve.
If you would like additional details on this, please go to the www.MiraiBio.com website.
The presentation The Calculations at the link shown below provides a much more in-
depth look at these issues:
http://www.MiraiBio.com/online-demo/online-demos.html
Going back to the exercise, in the plate above, if you wanted to save your work at this
point, you would select File → Save.
Note: When we read in a .csv file from the Luminex machine into MasterPlex QT,
the .csv file does not get altered. When we save the work we’ve done in MasterPlex QT,
it gets saved as an .mlx project file, leaving the original .csv file unaltered.