Luminex MasterPlex QT Tutorial Manual - Manual (Page 11)

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Note for the IL-10 analyte, some of the wells show a value <26.47. If we go to the drop-
down pick-list, and select Standard/Independent Values, we see that the lowest known
standard concentration for this analyte is 26.47.
So, what does <26.47 mean here? For 4-parameter logistic and 5-parameter logistic
curve models, when the best-fit curve is generated based on the data, there is a lower
limit for the Median Fluorescent Intensity (MFI) values that the model can use to
calculate an estimated concentration for a sample. When a sample has an analyte whose
MFI value is below this lower limit, the model cannot be used to calculate the estimated
concentration. However, if the MFI value for an analyte is below the MFI value for the