Luminex xMAP Antibody Coupling Kit - Manual (Page 21)

Page 17 of 20
Troubleshooting
This troubleshooting guide may be helpful in solving problems that may arise. For more
information, call Technical Support toll free at 1-877-785-BEAD (2323). Outside of U.S.
and Canada, call +1 512-381-4397. Send e-mail inquiries to: [email protected].
Problem Possible Cause(s) Recommendations
Low Bead
Count
Uncoupled
microspheres tend to
stick to the inner
surfaces of some
tubes
Make sure to use tubes supplied in this
kit only.
Microspheres were
lost during washes
Use the recommended magnetic
separator to perform all washes. See
Recommended Material list.
Use the transfer pipette to remove all
supernatant during washes. The
microsphere pellets are loose and
using a pipettor could disturb the bead
pellet.
Incorrect probe height
adjustment
Adjust probe height according to the
Luminex
®
200
™
, FLEXMAP 3D
®
or
MAGPIX
®
user manual.
Incorrect protocol set-
up on the Luminex
instrument
Make sure correct microsphere
regions are selected based on your
particular custom designed assay.