Storage and stability, Necessary materials and equipment – Luminex xMAP Antibody Coupling Kit User Manual
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Storage and Stability
Photosensitive microspheres should be protected from light at all times. All kit
components are to be stored at 2-8ºC. The EDC reagent, in its original packaging, may be
stored at -20°C to ensure the longest shelf-life possible. All other components should
never be frozen.
IMPORTANT: Due to the instability of the EDC reagent in solution, it should
always be stored in its original sealed packaging until needed and discarded after
one use. Reconstituted EDC should never be stored and reused.
All components are guaranteed up to the expiration date found on the label; when stored
in their original packaging and as specified in this manual.
The stability of coupled microspheres is dependent upon several factors; including the
protein stability and composition, aseptic processing conditions, presence of
preservatives, storage buffer, storage temperature conditions etc. However, stability
studies have shown that antibody coupled microspheres, stored in appropriate storage
buffer, are stable over a period of 18 months.
Necessary Materials and Equipment
There are various materials and pieces of equipment that are required for microsphere
coupling which are not included with this kit.
Required Equipment
•
Luminex xMAP instrument: MAGPIX, LX100/200, or FLEXMAP 3D
•
Luminex xPONENT software
•
Luminex MagPlex Microspheres (Cat# MC1XXXX-01, -04, & MC10XXX-ID)
NOTE: Visit
microspheres and guide-
lines for selecting the right regions for your instrument.
•
Luminex Instrument Performance Calibration and Verification Kits
•
Luminex Instrument Sheath Fluid (LX100/200 & FLEXMAP 3D) or Drive Fluid
(MAGPIX)
•
Magnetic Separator
Other Necessary Equipment
•
Tube Rotisserie or Rotator
•
Water-bath sonicator