Luminex xMAP Antibody Coupling Kit - Manual (Page 20)

Page 16 of 20
8. Mix the reactions gently by pipetting up and down several times with a pipettor.
9. Cover the plate and incubate for 30 minutes at room temperature on a plate shaker.
10. Clip the plate in place on the Luminex Magnetic Plate Separator and rapidly and
forcefully invert over a biohazard receptacle to evacuate the liquid from the wells.
NOTE: For information on the MagPlex Manual Wash Method, please visit:
http://www.luminexcorp.com/Products/ReagentsMicrospheres/MAGPLEX-
MICROSPHERES.
11. Wash each well with 100 μL of PBS-1% BSA by gently pipetting up and down
several times with a pipettor, and remove the liquid by using the procedure
described in the previous step.
12. Repeat the previous wash step once.
13. Resuspend the microspheres in 100 μL of PBS-1% BSA by gently pipetting up and
down several times with a pipettor.
14. Analyze 50-75 μL on the Luminex analyzer according to the system manual. An
example of typical results is shown below.