Carolina 3M Petrifilm Yeast & Molds Count Plates - Manual (Page 5)

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6. Gently apply pressure on the spreader to distribute the inoculum over
a circular area. Do not twist or slide the spreader (fig. 6).
Figure 6
7. Lift the spreader. Wait at least 1 minute for the gel to solidify (fig. 7).
Figure 7
8. Incubate plates with the clear side up in stacks of up to 20 plates.
Incubation time and temperature will vary according to the application
and equipment available.
9. Colonies on Petrifilm plates can be counted on a standard colony counter
or other light source (fig. 8). Yeast colonies will be small with defined
edges, and will be pink-tan to blue-green because of the indicator dye in
the medium. Mold colonies will be large, diffuse, and variable in color.
Figure 8