Carolina 3M Petrifilm Yeast & Molds Count Plates - Manual (Page 2)

Description
The 3M™ Petrifilm™ Yeast and Mold Count (YM) Plate is a ready-made
culture medium system that contains nutrients supplemented with
antibiotics, a cold-water-soluble gelling agent, and an indicator dye that
makes colonies easier to see. Petrifilm plates are manufactured with a grid
background to facilitate counting colonies. Petrifilm YM plates can be used
in place of standard fungal nutrient media such as Potato Dextrose Agar in
many applications, as described below:
• Petrifilm plates can be hydrated with a culture or a dilution of a culture for
counting the viable organisms present. See Method A.
• Petrifilm plates can be hydrated first with water or buffer, then inoculated
by swabbing, streaking, or touching to surfaces. See Method B.
• Cells can be removed from colonies growing on Petrifilm plates and used
to inoculate additional cultures or for staining.
• Experimental results on Petrifilm plates can be converted into electronic files
by scanning the plates on a standard computer-linked scanner. Do not open
plates to scan them.
Petrifilm plates were developed for use in the food and beverage industry.
They have been certified for official analyses in many countries. For more
information about these applications, see the 3M page on the World Wide
Web at www.3m.com.
Storage
Refrigerate unopened packages at
≤
8ºC (
≤
46ºF). Use before expiration date
on package.
To seal an opened package, fold the end over and tape it shut (fig. 1). Keep
resealed packages at room temperature and less than 50% relative humidity.
Do not refrigerate opened packages. Use plates from the opened package
within one month after opening.
Figure 1
©1999 Carolina Biological Supply Company Printed in USA
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