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Using spectrasuite, Using ooibase32 – Ocean Optics RSS-VA User Manual

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RSS-VA Installation and Operation Instructions

134-00000-000-01-201010

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Using the RSS-VA with Spectrometer Operating Software

Standard files are included with your Ocean Optics spectrometer operating software. These text files
have a *.STANDARD file extension.

Using SpectraSuite

Procedure

Follow the steps below to set up the software for use with the RSS-VA with SpectraSuite:

1. Start the SpectraSuite software, if necessary.

2. Set up your experiment as usual by choosing the appropriate integration period (so the signal-

to-noise will be high but the spectra or samples won’t be off scale). Choose scans to average
and box car smoothing to obtain the desired signal-to-noise ratio.

3. Store the dark and reference spectra and switch to %R (or %T) scales. SpectraSuite will make

your graph read 100% +/- random noise across the spectra.

4. Click on the

icon. The wizard asks for the location of the standard file. The file format

must be exactly as expected or SpectraSuite won’t be able to load it properly. Once you load
the file, the contents of the file header are shown as well as a graph of the reflectivity values.

5. Click the Apply button to make your stored reference read the values from the file,

interpolated to the wavelength at each pixel. You can take a new Reference spectrum at any
time without having to go through the wizard again.

See your SpectraSuite manual for more information about performing a reflectance experiment.

Using OOIBase32

Procedure

Follow the steps below to set up the software for use with the RSS-VA with OOIBase32:

1. Start the OOIBase32 software, if necessary.

2. Select Spectrum | Configure Standard Selection from the OOIBase32 menu bar. The

Configure Standard Selection dialog box appears.

3. Enable the check box next to the Enable Standard Correction option, and then enter the AOI

setting on the RSS-VA into the Reflection AOI box.

4. Navigate to and select the standard file in the OOIBase32\Standards directory. The standard

information is loaded into OOIBase32.

5. Click the OK button to store these inputs and close the dialog box.

6. Place the STAN-SSH or STAN-SSL standard on the sample port. Ensure that the standard is

mounted properly on the 3-point mount and that it is not tilted.

7. Store a reference spectrum in OOIBase32.

8. Turn the light source off and store a dark spectrum in OOIBase32. The system is now

normalized.