Saving the new calibration coefficients: usb mode – Ocean Optics QE65 Pro User Manual

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A: Calibrating the Wavelength of the QE65 Pro

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7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at

the value for R squared. It should be very close to 1. If not, you have most likely assigned one of
your wavelengths incorrectly.

Keep these values at hand.

Saving the New Calibration Coefficients: USB

Mode

Ocean Optics programs wavelength calibration coefficients unique to each QE65 Pro onto an EEPROM
memory chip in the QE65 Pro.

You can overwrite old calibration coefficients on the EEPROM if you are using the QE65 Pro via the
USB port.

►

Procedure

To save wavelength calibration coefficients using the USB mode, perform the following steps:

1. Ensure that the QE65 Pro is connected to the PC and that you have closed all other applications.

2. Point your browser to

and

scroll down to Microcode. Select USB EEPROM Programmer.

3. Save the setup file to your computer.

4. Run the Setup.exe file to install the software. The Welcome screen appears.

5. Click the Next button. The Destination Location screen appears.

6. Accept the default installation location, or click the Browse button to specify a directory. Then,

click the Next button. The Program Manager Group screen appears.

7. Click the Next button. The Start Installation screen appears.

8. Click the Next button to begin the installation. Once the installation finishes, the Installation

Complete screen appears.

9. Click the Finish button and reboot the computer when prompted.

10. Navigate to the USB EEPROM Programmer from the Start menu and run the software.

11. Click on the desired QE65 Pro device displayed in the left pane of the USB Programmer screen.

12. Double-click on each of the calibration coefficients displayed in the right pane of the USB

Programmer screen and enter the new values acquired in Steps 5 and 6 of the

section in this appendix.

13. Repeat Step 12 for all of the new values.

14. Click on the Save All Values button to save the information, and then Exit the USB Programmer

software.

The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
QE65 Pro.