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Appendix a, Calibrating the wavelength of the qe65 pro, Overview – Ocean Optics QE65 Pro User Manual

Page 19: About wavelength calibration, Calibrating the, Wavelength of the qe65 pro

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Appendix A

Calibrating the Wavelength of

the QE65 Pro

Overview

This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer
is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a
function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the
QE65 Pro.

About Wavelength Calibration

You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial:

λ

p

= I + C

1

p + C

2

p

2

+ C

3

p

3

Where:

λ

= the wavelength of pixel p

I = the wavelength of pixel 0

C

1

= the first coefficient (nm/pixel)

C

2

= the second coefficient (nm/pixel

2

)

C

3

= the third coefficient (nm/pixel

3

)

You will be calculating the value for I and the three Cs.