Hoefer SE1200 - Manual (Page 23)

•
p14
problem solution
Gel dries too slowly or not at all
Gel dries unevenly
Rotate each frame 180° (front-to-back) every
half hour.
Check the gel or stack Reduce the amount of glycerol to 0.5 to 2%.
Check that you are not using two sheets of Mylar.
At least one sheet must be cellophane.
Check the heater Make sure the indicator lamp lights at the low hear
or high hear settings. If it doesn’t light or the heater
does not heat, the safety switch may be tripped.
Check that the front and back of the dryer are unob-
structed and wait a few minutes for the dryer to cool,
then resume heating.
Gel turns cloudy or white
Gels ≥0.75 mm thick with
Reduce the percentage of ethanol to 30%.
acrylamide ≥10% T Do not soak the gel for more than 2 hours in the
ethanol/glycerol dehydration solution.
After Coomasie Blue staining, be sure to use final
destain of 7% acetic acid and 5% methanol.
Dryer does not function Check that the power switch is on.
Check that the power receptacle is properly grounded
and in working condition.
Check the fuses and replace, if necessary
If the dryer overheats, make sure air can flow freely
by checking that both the front and back are not
obstructed. If the dryer overheats more than once in
the same day, call Hoefer, Inc.