Hoefer TE42 - Manual (Page 31)

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p23
EDTA solution a
(0.5 M EDTA, pH 8.0, 100 ml)
Na
2
EDTA·2H
2
O (FW 372.2) 0.5 M 18.6 g
Dissolve in 70 ml distilled water. Adjust to pH 8.0 with 10 M
NaOH (approx. 5 ml), then add distilled water to 100 ml.
DNA transfer buffer, 10X
(10X Tris-borate-EDTA (TBE) a , pH ~8.2, 1 liter)
Tris (FW 121.1) 900 mM 109.0 g
Boric acid (FW 61.83) 900 mM 55.6 g
EDTA solution (0.5 M, pH 8.0) 20 mM 40.0 ml
Distilled water to 1.0 liter. Do not adjust pH.
Dilute to 1X before use to yield 90 mM Tris, 90 mM boric acid,
and 2 mM EDTA.
This dilution is commonly used, but dilutions down to 0.1X
may be used should it be necessary to decrease the amount
of current in the system in order to control overheating.
RNA transfer buffer, 10X
(10X Tris-acetate-EDTA (TAE) b , pH ~8.4, 1 liter)
Tris (FW 121.1) 400 mM 48.4 g
Acetic acid, glacial (~17.4 M) ~200 mM 11.4 ml
EDTA solution (0.5 M, pH 8.0) 10 mM 20.0 ml
Distilled water to 1.0 liter. Do not adjust pH.
Dilute to 1X before use to yield 40 mM Tris, ~20 mM acetate,
and 1 mM EDTA.
This dilution is commonly used, but dilutions down to 0.1X
may be used should it be necessary to decrease the amount
of current in the system in order to control overheating.
a Current Protocols in Molecular Biology (1993), A.2.1.
b Sambrook, J., and Russell, D.W. (2001) Molecular Cloning: A Labora-
tory Manual, A1.17.