Hoefer TE42 - Manual (Page 15)

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Close the cassette and press lightly to lock the tabs.
The assembled cassette should hold the gel in firm
contact with the membrane without squeezing the
gel. If the stack seems loose, add sheets of blot-
ting paper; if the stack seems tight, replace the
top sponge (above the gel) with a sheet of blotting
paper. If you remove the bottom sponge (below the
membrane), substitute at least two sheets of blotting
paper to create space between the membrane and the
cassette panel.
one 3 mm sponge for gels
>1.5 mm
—OR—
one 6 mm sponge for gels
≤1.5 mm.
blotting paper
blotting paper
one 3 mm sponge
gel
membrane
Fig 2. Transfer stack assembly. The
stack is oriented so that negatively
charged molecules migrate toward
the grey anode (+).
Important! Do not overstuff the
cassette.
Note: Try to place the gel correctly
the first time because proteins
may begin to transfer immediately;
once transfer has begun, moving
the gel will distort results or cause
“shadow bands” on the blot.
Assemble the cassette in a tray containing transfer
buffer about 3 cm deep.
The cassette panels are color coded:
black (top) = cathode side grey
(bottom) = anode side