Hoefer TE42 - Manual (Page 12)

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p4
Operating instructions
Perform the transfer as soon as possible after
electrophoresis to minimize band sample diffu-
sion. Each step is described below.
Prepare the buffer
Prepare a minimum of 5 liters of the appropriate
transfer buffer. Chill before use if possible.
Prepare the unit
1
Rinse the transfer tank and cassettes with
distilled water.
2
Active cooling is optional but strongly recommended. If
no active cooling will be used, go to step 3.
Note: Connect the heat exchanger to a circulator
bath such as the RCB20-PLUS.
Circulate only water or 50/50 water/ethylene glycol
to prevent damage to the unit.
The circulator pump must not generate a pressure
greater than 0.7 bar (10 psi) above atmospheric
pressure.
Set the temperature to 10 °C or higher if circulating
only water. If using 50/50 ethylene glycol/water, the
temperature can be set lower.
Start the circulator bath at the same time as
the transfer.
Note: Refer to the Electrotransfer
Notes section for a discussion of
membranes and buffers.
Note: For quick and easy connec-
tions, install Quick-fit coupler
fittings with valves in the line.