Hoefer TE42 - Manual (Page 13)

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p5
TE42
Lower the heat exchanger (ordered separately, or use
the heat exchanger supplied with the Hoefer SE600
Gel Electrophoresis Unit if you have one) into the
lower chamber, fitting the ports into the notches in
the rim. Prepare two lengths of 10–12 mm i.d. (3/8–
1/2") vinyl or silicone tubing for the cooling circuit
and skip to “Attach tubing” below.
TE62
First attach tubing to the red pressure relief valve
between the water inlet and outlet ports and insert
the free end into the bath or other container or drain
to catch any pressure relief overflow. Prepare two
lengths of 9 mm (3/8") vinyl or silicone tubing and
see “Attach tubing” below for instructions on fitting
it to the ports of the heat exchanger in the base of
the unit.
Attach tubing
Slide hose clamps (4 total) onto each end of two
lengths of tubing. Attach one end of each length of
tubing to a heat exchanger port. Attach the free ends
of each length of tubing to the circulator bath ports;
one to the inlet and the other to the outlet. Secure the
connections with the hose clamps.
3
Place ( do not drop) a magnetic stirring bar in the
buffer tank. (Dropping objects onto the alumina plate
in the TE62 may cause the plate to crack.) Set the
unit onto a magnetic stirrer. Fill transfer buffer to the
“Start fill level” line. (This requires approximately
3.8 liters.) Set the stirrer to low-medium, which
creates buffer circulation without forcing buffer
through assembled cassettes.
Note: The relief valve opens if the
pressure in the heat exchanger
exceeds 0.7 bar (10 psi) above
atmospheric pressure.
Note: For quick and easy connec-
tions, install Quick-disconnect
fittings with valves.
Note: Even if no cooling is
required for your system, the
buffer should be circulated with a
stirrer to avoid buffer depletion at
the electrodes.