Hoefer HE33 - Manual (Page 21)

• p13
2. 10X Tris-acetate-EDTA (TAE) stock buffer †
(0.4 M Tris, 0.2 M acetic acid, 10 mM EDTA,
pH ~8.4, 1000 ml)
Tris base (FW 121.1) 0.40 M 48.4 g
Acetic acid (99.5%) 0.20 M 11.4 ml
EDTA solution
(0.5 M, pH 8.0, soln. 3)
0.01 M 20.0 ml
Deionized H
2
O to 1000.0 ml
Stir. Do not adjust pH. Dilute to 1X before use to
yield 40 mM Tris base, 20 mM acetic acid,
and 1 mM EDTA.
3. EDTA solution (ethylenediamine tetraacetic acid) †
(0.5 M, pH 8.0, 100 ml)
Na
2
EDTA·2H
2
O, (FW 372.2) 0.5 M 18.6 g
Deionized H
2
O to 70.0 ml
NaOH (10 M) to pH 8.0 ~5.0 ml
Deionized H
2
O to 100.0 ml
† Modified from Sambrook, J., Molecular Cloning: A Laboratory Manual,
p. B.23 (1989). See also Current Protocols in Molecular Biology,
p. A.2.1 (1993).