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Care and maintenance, Cleaning, Replacing foam pads – Hoefer HE33 User Manual

Page 17: Replacing the electrode

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Care and maintenance

Cleaning

After each use, clean the unit with a mild deter-
gent and water, rinse thoroughly with distilled
water, and allow to air dry. Never use abrasive
cleansers. Do not expose the unit to solutions
or vapors of aromatic or halogenated hydrocar-
bons, ketones, esters, alcohols (over 30%), or
concentrated acids (over 25%).

To reduce DNase and RNase contamination,
soak the buffer chamber or casting kit for 10
minutes in a 3% hydrogen peroxide (H

2

O

2

)

solution, then rinse thoroughly with DEPC-
treated, autoclaved, deionized water. (Sambrook,
et al. 1:7:40)

Replacing foam pads

Remove worn foam pads. Peel off the adhesive
cover on a new foam pad. Align the pad so that it
will rest on the bottom of the tray along a short
(7 cm) side, adhesive side toward the inside wall,
and then press it in place. Repeat with second
pad on the wall opposite the first pad.

Replacing the electrode

It is recommended that electrodes be replaced
only by Hoefer technicians. Call your local
representative for advice.

Important! Never autoclave
any component of the
electrophoresis unit or
casting kit.