Hoefer HE33 - Manual (Page 12)

• p4
Prepare solutions

Prepare 250 ml of running buffer. (Refer to page 12
for recipes of commonly used electrophoretic running
buffers.)

Prepare the sample loading buffer. (Refer to page 14
for a recipe and a table of volume requirements for
each comb size.)

Prepare approximately 7 ml agarose solution per mm
of gel thickness. (For example, a 3-mm gel requires
0.3 cm × 7 cm × 10 cm = 21 ml)
Dissolve agarose in running buffer, heat according
to instructions accompanying the agarose, and allow
the solution to cool to 50 °C before pouring into the
casting tray.
Optional: Add 0.5 µg/ml ethidium bromide to the gel
solution to observe separation during electrophoresis.
Caution: Ethidium bromide is a
known mutagen. Always wear
gloves when handling.
Fig 2. Gel casting kit.
Approach the foam pad with one
end of the running tray and then
gently press the tray edge against
the pad, compressing it enough
to allow the opposite end of the
running tray to drop fully into the
casting tray before sealing against
the foam pad.
UV-transparent
running tray
(cast the gel on this
tray, then transfer
gel to the horizontal
unit base for
electrophoresis.)
gel casting
tray foam
pads (2)