Prepare the monomer solution – Hoefer SE215 User Manual
Page 8
Prepare the monomer solution
1
Prepare enough monomer solution to fill all sand-
wiches to the level of the notched plate (or lower
if a stacking gel or first dimension IPG strip will be
added —see step 2). The formula below allows extra
solution to fill the space between the sandwich and
the chamber.
•
p6
Table 2: Variables for calculating monomer volume
gel caster
area X cm
2
extra vol. required (Y ml)
SE215
60
11
SE235
80
8
SE275
60
5
Monomer = area X × spacer
×
total gel
+
extra chamber
vol (ml)
(cm
2
)
thickness (cm) sandwiches vol (Y ml)
An example volume calculation for the SE215 for 10 sandwiches, each with a thickness of
1.5 mm follows: From Table 2, the area variable for the SE215 is 60 and the extra volume
variable is 11 ml.
(60 cm
2
) (0.15 cm) (10 sandwiches) + 11 ml extra volume required = 101 ml