Hoefer SE215 - Manual (Page 5)

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Single-concentration gels — Plug the red inlet port
near the bottom of the faceplate with the black vinyl
cap included with the unit. Check that the two small
semi-circular and one large triangular rubber filler
plugs, which reduce the amount of solution needed
to fill the chamber, are properly seated in the bottom.
The crosswise groove in the plug distributes the mono-
mer solution to all gel sandwiches so that they fill to
the same level simultaneously.
Gradient gels — Remove the large triangular plug.
Check that the two smaller rubber filler plugs are
properly seated in the bottom of the chamber. Refer
to page 9 for gradient casting instructions.
Construct the gel sandwich stack and
load into caster
1
Construct each gel sandwich: For each sandwich,
choose one notched alumina plate, one rectangular
glass plate and two spacers. Use the same size spac-
ers for each sandwich.
Lay the notched plate on a flat surface, place one
spacer along each edge so that it aligns with the
notch. Fit a glass plate onto the spacers as shown.
The stem of the T-shaped spacers fits between both
plates. The top of the T forms the edge of the gel
sandwich, (Fig 2).
(Alternatively, assemble the sandwich while it is in
a standing position.)
2
Pick up each sandwich and align both sides with the
spacers and also the bottom on a flat surface, (Fig 3).
Fig 2. Location of T-shaped
spacers on notched plate.
Fig 3. Align bottom edges of plates
and spacers carefully.