Hoefer SE215 - Manual (Page 17)

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p15
problem solution
Sample wells damaged or leak Remove air pockets before inserting combs. Slide comb
into solution at an angle. If comb must be removed,
add more monomer solution before reinserting the comb.
Allow the gel to set for a minimum of 1 hour.
Remove the comb at a slight angle and very slowly to
prevent the gel from becoming damaged.
Degas stacking gel solution.
Increase catalysts up to 0.1% v/v TEMED,
0.1% v/v APS.
Gel sandwiches separate Place wax paper between sandwiches in the stack and
in the back of the caster so that sandwiches separate
easily from each other. (This reduces any tendency
for plates to stick to each other instead of to the gel.)
Also, place a polycarbonate sheet on the top of the
stack to prevent a vacuum from forming between the
glass and acrylic face plate.
Gradient gels — uneven layering Add sucrose (15% final concentration) or glycerol
(25% final concentration) to the high percent mono-
mer solution.
Add a small amount of bromophenol blue to the heavy
solution to track the gradient formation.
Decrease the pump rate.