Hanna Instruments HI 83746 User Manual

Page 7

MEASUREMENT

Note: A single blank vial may be used more than once; the blank vial is stable up to one week if
stored in a dark place at room temperature. Always use the same lot of reagents for blank and
samples. For most accurate measurement run a blank for each set of mesurement.
Note: If the expected RS concentration is above 50.00 g/L (for example for must analysis), it is
recommended to dilute the sample 4 or 5 times with water.

• Remove the cap from two vials HI 83746A-0

Fehling Solution A.

• Use the HI 731341 1000 µL automatic pipette to add

exactly 1 mL of HI 83746B-0 Fehling Solution B to
each vial.
For a correct use of the automatic pipette please
follow the related Instruction Sheet.

• Use the HI 731340 200 µL automatic pipette to

add exactly 200 µl of wine sample to one vial
(Sample) and 200 µL of deionized water to the
other vial (Blank).

• Replace the cap and invert the vials several times to

mix. Wipe the vials thoroughly with a lint-free
cloth.

SAMPLE PREPARATION FOR RED WINE

To remove interference of phenols, samples of Red
Wine must be treated with activated carbon.

• Fill one cuvet with 10 mL of Red Wine.

• Add 2 spoons of HI 93703-59 Charcoal to the

cuvet.

• Swirl the cuvet for 2 minutes to mix. Then

wait for 3 minutes.

• Fold a filter disc twice as shown in the figure.

Separate one side from the other three to form a
cone. Insert the folded filter disc in the funnel.

• Filter the treated wine into an empty cuvet. This

is the wine sample.

MEASUREMENT PROCEDURE

wine
sample

deionized
water

Sample

Blank

• Preheat the Hanna Reactor HI 839800 to 105 °C

(221°F). For a correct use of the reactor follow the
Reactor Instruction Manual.
Use of the optional HI 740217 safety shield is
recommended.
Do not use an oven or microwave.